Welcome to the DTU Chemistry course Experimental Chromatographic Purification of Recombinant Proteins - 26125.
You will be working in pairs. Each day you will spend half a day in the lab and half a day studying the theoretical concepts, preparing for the experimental exercises, and evaluating your results. The course schedule is as follows.
Active participation in all exercises is required (in other words attendance every day from 8.00 am to 5 pm is compulsory) in order to attend the final oral exam.
Daily Schedule X-1 team:
Abbreviations:
RA: Risk assessment
IEX: Ion-exchange
HR-IEX: High-resolution IEX
HIC: Hydrophobic interaction chromatography
AC:Affinity chromatography
GF: Gel filtration
UF: Ultrafiltration
MBP: maltose binding protein
TPH: Tryptophan hydroxylase
FF: Fast flow
FXD: Ferredoxin
CIP: Cleaning in place
SDS-Page: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis